There are several benefits to chronic electrical stimulation. The rationale, however, is always the same: treat the cells in a manner consistent with their native environment.
Like using proper maintenance media and incubation, chronic culture pacing will help induce and/or maintain phenotype over time.
The C-Pace EP can be configured with up to 8 channels and electrode assemblies for common 4-, 6-, 8-, 12-, and 24-well culture dishes are available.
Our chronic pacers have been used to culture a variety of cells that are electrically coupled in vivo, including smooth and striated muscle. They’ve also been used to facilitate the differentiation of stem cells. Looking for a better way to culture cells? Try the C-Pace EM.
C2C12 myotubes without pacing after several days of culture in differentiating medium, stained with DAPI and alpha-actinin. Images courtesy of Marloes Langelaan, Technical University Eindhoven, The Netherlands
C2C12 myotubes chroniclaly paced for several days of culture in differentiating medium, stained with DAPI and alpha-actinin. Images courtesy of Marloes Langelaan, Technical University Eindhoven, The Netherlands